Homozygous mutation of VPS16 gene is responsible for an autosomal recessive adolescent-onset primary dystonia

نویسندگان

  • Xiaodong Cai
  • Xin Chen
  • Song Wu
  • Wenlan Liu
  • Xiejun Zhang
  • Doudou Zhang
  • Sijie He
  • Bo Wang
  • Mali Zhang
  • Yuan Zhang
  • Zongyang Li
  • Kun Luo
  • Zhiming Cai
  • Weiping Li
چکیده

Dystonia is a neurological movement disorder that is clinically and genetically heterogeneous. Herein, we report the identification a novel homozygous missense mutation, c.156 C > A in VPS16, co-segregating with disease status in a Chinese consanguineous family with adolescent-onset primary dystonia by whole exome sequencing and homozygosity mapping. To assess the biological role of c.156 C > A homozygous mutation of VPS16, we generated mice with targeted mutation site of Vps16 through CRISPR-Cas9 genome-editing approach. Vps16 c.156 C > A homozygous mutant mice exhibited significantly impaired motor function, suggesting that VPS16 is a new causative gene for adolescent-onset primary dystonia.

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2016